Coding

Part:BBa_K4192011:Design

Designed by: Lehan Dong   Group: iGEM22_CAU_China   (2022-09-29)


gacA from Pseudomonas fluorescens str. 2P24


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 316
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The effect of this gene is not stable, and increasing its expression can effectively improve the production of biofilm. However, there is a large difference between the parallel controls. It can play a better role when co-expressed with other regulatory factors such as cdg (BBa_K4192010).



Source

It comes from the genome of Pseudomonas fluorescens 2P24.

References

[1]Seaton, Sarah C et al. “Pleiotropic effects of GacA on Pseudomonas fluorescens Pf0-1 in vitro and in soil.” Applied and environmental microbiology vol. 79,17 (2013): 5405-10. doi:10.1128/AEM.00819-13